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anti total 1 integrin  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti total 1 integrin
    Anti Total 1 Integrin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+total+1+integrin/anti-integrin+beta-1/bio_rxiv__2024__11__05__621850-418-53-57
    Average 92 stars, based on 18 article reviews
    anti total 1 integrin - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    other:

    Article Title: A Proximity MAP of RAB GTPases
    Article Snippet: Antibodies used throughout the study were anti-MYC (1:500; 71D10; Cell Signaling Technology), anti-transferrin receptor (1:100; D59X; Cell Signaling Technology), anti-EEA1 (1:500; C45B10; Cell Signaling Technology), anti-HA (1:500; C29F4; Cell Signaling Technology), anti-TGN46 (1:100; Clone 2F7.1; Genetex), anti-CIMPR (1:100; MCA2048; BioRad), anti-LAMP1 (1:15; 1D4B; DHSB), anti-KDEL (1:200; EPR12668; Abcam), anti-TOM20 (1:200; 612278; BD Biosciences), anti-total ≥1-integrin (1:100; P5D2; DSHB), anti-active ≥1-integrin (1:100; clone 12G10; Millipore Sigma).



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    A Representative images of MEFs of each genotype co-stained with a total <t>β1-integrin</t> antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.
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    A Representative images of MEFs of each genotype co-stained with a total <t>β1-integrin</t> antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.
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    A Representative images of MEFs of each genotype co-stained with a total <t>β1-integrin</t> antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.
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    A Representative images of MEFs of each genotype co-stained with a total <t>β1-integrin</t> antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.
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    A Representative images of MEFs of each genotype co-stained with a total <t>β1-integrin</t> antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.
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    A Representative images of MEFs of each genotype co-stained with a total <t>β1-integrin</t> antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.
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    Image Search Results


    A Representative images of MEFs of each genotype co-stained with a total β1-integrin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.

    Journal: Communications Biology

    Article Title: Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis

    doi: 10.1038/s42003-025-08294-3

    Figure Lengend Snippet: A Representative images of MEFs of each genotype co-stained with a total β1-integrin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.

    Article Snippet: Cells were incubated with primary antibodies goat anti-total β1-integrin (1:500, R&D Systems, AF2405) and rat anti-active β1-integrin 9EG7 (1:10, BD Biosciences, 550531) diluted in flow buffer for 1 h on ice followed by three washes with flow buffer.

    Techniques: Staining